Università degli Studi di Napoli - Federico II
Department: Department of Biological Sciences
Via Mezzocannone 16 - Napoli - 80134
Rif: Prof. Carlo Pedone
Tel. 081-2536651 - Email: - carlo.pedone@unina.it
Web: http://www.unina.it
CV: Download Cv

---

Participants
Personale DSB : Ettore Benedetti, Giancarlo Morelli, Stefania Galdiero, Diego Tesauro, Alessandra Romanelli, Daniela Marasco, Paolo Abrescia, Luisa Cigliano. Personale IBB-CNR : Luigi Vitagliano, Rita Berisio, Katia D' Ambrosio, Giuseppina De Simone, Luciana Esposito, Michele Saviano, Luca Domenico D' Andrea, Stefania De Luca, Laura Zaccaro, Giovanni Rovello, Menotti Ruvo, Sonia Di Gaetano, Nina Dathan, Simona Maria Monti, Rosanna Palombo, Emilia Pedone, Gruppi associati: Gruppo diretto dal Prof. Gennaro Marino, Dipartimento di Chimica Organica e Biochimica (DCOB)-Universita' degli Studi di Napoli "Federico II" Gruppo diretto dal Prof. Gerry Melino, Universita' Tor Vergata di Roma Roma; Gruppo diretto dal Prof. Guidalberto Manfioletti Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Universita' di Trieste.

Current Activities

 

• Characterization of proteins involved in cancer  

• Identification and characterization of proteins involved in infectious diseases

• Identification and characterization of proteins involved in pathologies of the nervous system

• Characterization of protein-protein interactions by the identification of protein functional fragments (fragmentomics)

 

---

Facilities

 

 

 

• Synthesis and characterization of peptides and nucleotides

• Structural biology (Cristallography, NMR, Bioinformatics)

• Molecular design

• Protein expression and purification 

• Mass spectrometry

• High throughput  screening

• Physico-chemical characterizaion of biomolecules (Fluorescence, CD, EPR)

• Surface Plasmon Resonance

 

 

 

 

 

 

 

 

 

 

 

 

---

Instrumentation

 Peptide and nucleotide synthesis 

• Automatic synthesizer Advanced ChemTEK
• Automatic synthesizer Applied Biosystem ABI 433
• Automatic synthesizer SYRO I Multisynthec
• Automatic synthesizer SOPHAS Zynsser
• Automatic synthesizer for DNA and PNA ABI

Purification of protein, peptides and nucleotides

• HPLC HP1050 
• HPLC Agilent 1200 
• HPLC preparative Varian Prepstar D81
• 2 HPLC analytic Hewlett Packard 1100
• HPLC analytic Shimadzu LCA10 
• 2 HPLC preparative Shimadzu LC8A
• HPLC analytic WATERS ALLIANCE
• 3 FPLC GE.

Biomolecule characterization and binding analysis

• 2D LC/MS ESI nanospray Bruker
• LC/MS LCQXPDECA Ion Trap Mass Spectrometer (Thermo)
• LC/MS System Single quadrupole MSQ (Thermo)
• SPR BIACORE
• SPR SENSI Q
• Cytofluorimeter 
• FRET 
• ELISA

Structural characterization (solution) 

• Varian 600 Mhz with cryoprobe
• Varian 400 MHz
• Circular Dichroism Jasco J715
• Spectrofluorimeter (Varian)

Structural characterization (solid state)

• Rotating anode diffractometer equipped with area detector for proteins (Bruker)
• Rotating anode diffractometer equipped with CCD for small molecules and proteins (Rigaku)
• Hamilton robot station for the screening of crystallization conditions

Drug design  and Biocomputing

• 16 processor cluster for parallel computing;
• 8 HP biprocessor workstation
• 6 SGI Octane2 Workstation for drug design

HTS screening

• Hamilton robot station for HTS screening
• Hamilton robot station for compound weighting, archiving and storage.
 

---